Selected article for: "gene expression and mean vector"

Author: Shapira, Assaf; Benhar, Itai
Title: Toxin-Based Therapeutic Approaches
  • Document date: 2010_10_28
  • ID: 00cf294x_30
    Snippet: Prostate cancer (PCA) is the most common cancer diagnosed in men and a leading cause of cancer deaths [367] . Normal and malignant prostate epithelia specifically express the kallikrein protease prostate-specific antigen (PSA), a serine protease with trypsin-and chymotrypsin-like activities that is responsible for liquifaction of semen [368, 369] . PSA gene regulatory regions are prime candidates to direct prostate-specific expression [370] [371].....
    Document: Prostate cancer (PCA) is the most common cancer diagnosed in men and a leading cause of cancer deaths [367] . Normal and malignant prostate epithelia specifically express the kallikrein protease prostate-specific antigen (PSA), a serine protease with trypsin-and chymotrypsin-like activities that is responsible for liquifaction of semen [368, 369] . PSA gene regulatory regions are prime candidates to direct prostate-specific expression [370] [371] [372] [373] . When placed under the transcriptional regulation of the PSA promoter, the diphtheria toxin A chain (DT-A) encoding gene which was delivered by an adenoviral vector showed strong inhibition of tumor growth in a PSA-producing prostate tumor xenograft mouse model while not affecting non-PSA producing tumor xenografts [339] . A PSA promoter-based lentiviral vector has also been used as a mean for DT-A gene delivery, leading to specific eradication of prostate tumor xenografts in nude mice following a single intratumoral injection. Repetitive injections were shown to inhibit the growth of recurrent tumors [374] .

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