Author: Zapata, Juan Carlos; Carrion, Ricardo; Patterson, Jean L.; Crasta, Oswald; Zhang, Yan; Mani, Sachin; Jett, Marti; Poonia, Bhawna; Djavani, Mahmoud; White, David M.; Lukashevich, Igor S.; Salvato, Maria S.
Title: Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate Document date: 2013_9_12
ID: 0epeljaf_14
Snippet: Gene expression profiling using cDNA microarrays Total RNA was isolated from PMBC samples using the Trizol method (Invitrogen, Carlsbad, CA) followed by a cleaning step with Rneasy mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions. Quality and quantity of all RNA samples were evaluated on the Agilent 2100 BioAnalyzer 116 system (Agilent Technologies, Palo Alto, CA) by looking at 18 & 28 s rRNA peaks and by the RIN (RNA.....
Document: Gene expression profiling using cDNA microarrays Total RNA was isolated from PMBC samples using the Trizol method (Invitrogen, Carlsbad, CA) followed by a cleaning step with Rneasy mini kit (Qiagen, Valencia, CA), according to the manufacturer's instructions. Quality and quantity of all RNA samples were evaluated on the Agilent 2100 BioAnalyzer 116 system (Agilent Technologies, Palo Alto, CA) by looking at 18 & 28 s rRNA peaks and by the RIN (RNA integrity number). High quality RNA was labeled and hybridized according to Affymetrix protocols using the GeneChip human genome U133 Plus 2.0 array (Affymetrix, Santa Clara, CA) and as described previously [24] . This chip covers the whole human genome using 54,000 probe sets representing approximately 22,000 genes.
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