Author: Pervushin, Konstantin; Tan, Edward; Parthasarathy, Krupakar; Lin, Xin; Jiang, Feng Li; Yu, Dejie; Vararattanavech, Ardcharaporn; Soong, Tuck Wah; Liu, Ding Xiang; Torres, Jaume
Title: Structure and Inhibition of the SARS Coronavirus Envelope Protein Ion Channel Document date: 2009_7_10
ID: 1e102wrc_48
Snippet: For the sample preparation in the presence of 16-doxyl stearic acid (16-DSA), the desired amount of 16-DSA was first dissolved in methanol. The aliquots of 16-DSA corresponding to 1 mM, 3 mM and 5 mM of 16-DSA in final NMR samples were added to an empty NMR tube and dried under a stream of dry N 2 gas. The NMR sample containing ETM/DPC was added to the NMR tube containing the dry 16-DSA and was left to equilibrate for a few hours. Gadodiamide (OM.....
Document: For the sample preparation in the presence of 16-doxyl stearic acid (16-DSA), the desired amount of 16-DSA was first dissolved in methanol. The aliquots of 16-DSA corresponding to 1 mM, 3 mM and 5 mM of 16-DSA in final NMR samples were added to an empty NMR tube and dried under a stream of dry N 2 gas. The NMR sample containing ETM/DPC was added to the NMR tube containing the dry 16-DSA and was left to equilibrate for a few hours. Gadodiamide (OMNISCAN; gadolinium chelated with 2-[bis[2-[(2-methylamino-2-oxoethyl)-(2-oxido-2-oxoethyl)amino]ethyl]amino]acetate, GE Healthcare) was used from a 0.5 M stock solution and was diluted to 1.5 mM.
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