Author: Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo-Yun; Li, Kui
Title: The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function Document date: 2019_6_28
ID: 1nr0hggt_13
Snippet: ZIKV MR766 strain (BEI Resources # NR-50065) and PRVABC59 strain (kindly provided by Brandy Russel, CDC, Fort Collins, CO) were propagated in C6/36 cells. For infections of various cell types, cells were inoculated with ZIKV in 2% FBS-containing DMEM at indicated multiplicities of infection (MOIs) for 1-2 h. Subsequently, viral inoculum was removed, and cells were washed three times in PBS, refed with complete culture medium and cultured for the .....
Document: ZIKV MR766 strain (BEI Resources # NR-50065) and PRVABC59 strain (kindly provided by Brandy Russel, CDC, Fort Collins, CO) were propagated in C6/36 cells. For infections of various cell types, cells were inoculated with ZIKV in 2% FBS-containing DMEM at indicated multiplicities of infection (MOIs) for 1-2 h. Subsequently, viral inoculum was removed, and cells were washed three times in PBS, refed with complete culture medium and cultured for the indicated time periods. Infectious virus titers in cell-free culture supernatants were determined by an endpoint dilution-based 50% tissue culture infective dose (TCID 50 ) assay in 96-well plates [37, 38] . Titration of ZIKV was performed on Vero cells, and cytopathic effect (CPE) was recorded and used for calculation of viral titers at 7 days post infection (dpi) by the Reed and Muench method [39] .
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