The discriminative capacity of soluble Toll-like receptor (sTLR)2 and sTLR4 in inflammatory diseases

ten Oever, Jaap; Kox, Matthijs; van de Veerdonk, Frank L; Mothapo, Khutso M; Slavcovici, Adriana; Jansen, Tim L; Tweehuysen, Lieke; Giamarellos-Bourboulis, Evangelos J; Schneeberger, Peter M; Wever, Peter C; Stoffels, Monique; Simon, Anna; van der Meer, Jos WM; Johnson, Melissa D; Kullberg, Bart-Jan; Pickkers, Peter; Pachot, Alexandre; Joosten, Leo AB; Netea, Mihai G

This study was part of a larger endotoxin trial registered at the ClinicalTrials.gov registry under the number NCT00783068 which was approved by the local ethics committee of the Radboud university medical center [14] . The 11 healthy male volunteers included in the present study provided written informed consent. Briefly, subjects were prehydrated during 1 h before LPS administration by infusion of 1.5 L 2.5% glucose/0.45% saline solution, followed by 150 ml/h starting at the time of LPS administration until 6 h afterwards and 75 ml/h until the end of the experiment. US Reference Escherichia coli endotoxin (LPS derived from E. coli O:113; Clinical Center Reference Endotoxin, National Institutes of Health, Bethesda, Md) was administered as an intravenous bolus (2 ng/kg). EDTA anticoagulated blood was collected from an arterial line.