Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_30
Snippet: gB Mutations That Impact Fusion ® BAC pQF297 was transfected into Vero cells expressing Cre recombinase by using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). The transfected cells were harvested 3 weeks after transfection, sonicated, and subsequently passaged on Vero cells to generate HSV-1 gB 3A stocks. gB 3A-1 and gB 3A-2 viruses were isolated from two separate transfections of pQF297, and both contain the same triple alanine mutation in gB,.....
Document: gB Mutations That Impact Fusion ® BAC pQF297 was transfected into Vero cells expressing Cre recombinase by using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). The transfected cells were harvested 3 weeks after transfection, sonicated, and subsequently passaged on Vero cells to generate HSV-1 gB 3A stocks. gB 3A-1 and gB 3A-2 viruses were isolated from two separate transfections of pQF297, and both contain the same triple alanine mutation in gB, as confirmed by sequencing. WT GS3217 virus was harvested at 6 to 7 days posttransfection. Viral DNA was sequenced by the Northwestern Genomic core facility.
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