Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site Document date: 2015_3_25
ID: 10p3mth2_56
Snippet: Using kinetic and single-molecule fluorescence data, three recent studies, utilising in vitro translation systems, have observed frameshifting occurring during translocation in the prokaryotic dnaX and infectious bronchitis virus elements on bacterial ribosomes [30] [31] [32] . While the elements examined here had modifications to give a comparatively high frameshift efficiency (> 70%), our proposed model of 'late' lower efficiency of 5-10% frame.....
Document: Using kinetic and single-molecule fluorescence data, three recent studies, utilising in vitro translation systems, have observed frameshifting occurring during translocation in the prokaryotic dnaX and infectious bronchitis virus elements on bacterial ribosomes [30] [31] [32] . While the elements examined here had modifications to give a comparatively high frameshift efficiency (> 70%), our proposed model of 'late' lower efficiency of 5-10% frameshifting in HIV shares some similarities with the proposed mechanism of −1 PRF in dnaX, in which the A site codon can be 'sampled' by tRNA with the ribosome in an abnormal rotated state [32] .
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