Author: Cooray, Samantha; Jin, Li; Best, Jennifer M
Title: The involvement of survival signaling pathways in rubella-virus induced apoptosis Document date: 2005_1_4
ID: 1i36lsj2_16
Snippet: To evaluate the role of PI3K-dependent signaling during RV infection, the effects of PI3K inhibitor LY294002 on the development of RV-induced apoptosis were examined, 12-96 hours p.i., by caspase activity assay, trypan blue exclusion staining, DNA fragmentation and light microscopy. (Fig. 3A-D) . RV-induced apoptotic signaling has been reported to occur between 12-24 hours p.i., with peak caspase activity occurring around 72 hours p.i. at a multi.....
Document: To evaluate the role of PI3K-dependent signaling during RV infection, the effects of PI3K inhibitor LY294002 on the development of RV-induced apoptosis were examined, 12-96 hours p.i., by caspase activity assay, trypan blue exclusion staining, DNA fragmentation and light microscopy. (Fig. 3A-D) . RV-induced apoptotic signaling has been reported to occur between 12-24 hours p.i., with peak caspase activity occurring around 72 hours p.i. at a multiplicity of infection (MOI) of 3 PFU/cell [6] . Fig. 3A shows that with a MOI of 4 PFU/cell the peak of RVinduced caspase activity occurs earlier at 60 hours p.i. When RV infection was carried out in the presence of LY294002, the maximum caspase activity increased by 53.9 % (P < 0.05) and occurred 12 hours earlier than with RV alone (Fig. 3A ).
Search related documents:
Co phrase search for related documents- blue exclusion staining and dna fragmentation: 1
- blue exclusion staining and light microscopy: 1
- blue exclusion staining and rv induced apoptosis: 1
- caspase activity and dna fragmentation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- caspase activity and PFU cell: 1, 2
- caspase activity and rv induced apoptosis: 1, 2, 3
- caspase activity and rv infection: 1, 2, 3
- dna fragmentation and infection multiplicity: 1
- dna fragmentation and light microscopy: 1, 2
- dna fragmentation and rv induced apoptosis: 1
- dna fragmentation and rv infection: 1, 2, 3
- infection multiplicity and light microscopy: 1
- infection multiplicity and MOI infection multiplicity: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81
- infection multiplicity and PFU cell: 1, 2, 3, 4, 5, 6, 7, 8, 9
- light microscopy and MOI infection multiplicity: 1
- light microscopy and rv induced apoptosis: 1
- light microscopy and rv infection: 1, 2
- MOI infection multiplicity and PFU cell: 1, 2, 3, 4, 5, 6, 7
- PFU cell and rv infection: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date