Selected article for: "accession genbank and long range"
Author: Antunes, Agostinho; Troyer, Jennifer L.; Roelke, Melody E.; Pecon-Slattery, Jill; Packer, Craig; Winterbach, Christiaan; Winterbach, Hanlie; Hemson, Graham; Frank, Laurence; Stander, Philip; Siefert, Ludwig; Driciru, Margaret; Funston, Paul J.; Alexander, Kathy A.; Prager, Katherine C.; Mills, Gus; Wildt, David; Bush, Mitch; O'Brien, Stephen J.; Johnson, Warren E.
Title: The Evolutionary Dynamics of the Lion Panthera leo Revealed by Host and Viral Population Genomics
  • Document date: 2008_11_7
    • ID: 095u8eaj_31
    Snippet: A total of 357 individuals were obtained across most of the lion range in Africa and Asia ( Figure 1A ; Table S1 ). Genetic variation among lion specimens was assessed using maternal (12S and 16S genes), paternal (SRY gene) and bi-parental autosomal (22 microsatelite loci, and the ADA and TF genes) markers (GenBank accession numbers: FJ151632-FJ151652). Analyses of mtDNA in Panthera species are complicated by the presence of a 12.5 kb mtDNA integ.....
    Document: A total of 357 individuals were obtained across most of the lion range in Africa and Asia ( Figure 1A ; Table S1 ). Genetic variation among lion specimens was assessed using maternal (12S and 16S genes), paternal (SRY gene) and bi-parental autosomal (22 microsatelite loci, and the ADA and TF genes) markers (GenBank accession numbers: FJ151632-FJ151652). Analyses of mtDNA in Panthera species are complicated by the presence of a 12.5 kb mtDNA integration into chromosome F2 [42] . Accordingly, mtDNA specific primers were designed for the 12S and 16S genes (Table S2 ) and we used long-range PCR amplification. We designed primers to amplify segments of the ADA (exon 10 and intron 10) and the TF (intron 3) genes (Table S2) , two of the most variable protein loci in lion populations [5] , localized on the domestic cat Felis catus chromosome A3p and C2q, respectively. The Y-chromosome SRY-39UTR gene was also amplified [43] .

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