Author: Wang, Qing S.; Jan, Eric
Title: Switch from Cap- to Factorless IRES-Dependent 0 and +1 Frame Translation during Cellular Stress and Dicistrovirus Infection Document date: 2014_8_4
ID: 0if5z3xp_9
Snippet: Capped reporter RNAs were obtained by in vitro transcription in the presence of cap analog [m 7 G(59)ppp(59)G] (Ambion) to GTP at a 5:1 ratio as previously described [31] . RNAs were purified by RNeasy (Qiagen), their integrity confirmed by denaturing agarose gel analysis, and RNA concentration determined using a Nanodrop ND-1000 spectrophotometer......
Document: Capped reporter RNAs were obtained by in vitro transcription in the presence of cap analog [m 7 G(59)ppp(59)G] (Ambion) to GTP at a 5:1 ratio as previously described [31] . RNAs were purified by RNeasy (Qiagen), their integrity confirmed by denaturing agarose gel analysis, and RNA concentration determined using a Nanodrop ND-1000 spectrophotometer.
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