Author: Hoffmann, Markus; Krüger, Nadine; Zmora, Pawel; Wrensch, Florian; Herrler, Georg; Pöhlmann, Stefan
Title: The Hemagglutinin of Bat-Associated Influenza Viruses Is Activated by TMPRSS2 for pH-Dependent Entry into Bat but Not Human Cells Document date: 2016_3_30
ID: 0ejhd9nw_8
Snippet: To investigate the roles of sialic acids and endosomal acidification in batFLUAV entry, we used recombinantly-produced, bacterial sialidase (Clostridium welchii, Sigma-Aldrich) and ammonium chloride (Sigma-Aldrich). For treatment, the cell culture supernatant was removed and the cells were washed with phosphate buffered saline (PBS) before culture medium containing water (negative control), ammonium chloride (50 mM) or different concentrations of.....
Document: To investigate the roles of sialic acids and endosomal acidification in batFLUAV entry, we used recombinantly-produced, bacterial sialidase (Clostridium welchii, Sigma-Aldrich) and ammonium chloride (Sigma-Aldrich). For treatment, the cell culture supernatant was removed and the cells were washed with phosphate buffered saline (PBS) before culture medium containing water (negative control), ammonium chloride (50 mM) or different concentrations of bacterial sialidase (1.5, 15 or 150 mU) was added. After 2 h of incubation at 37°C and 5% CO 2 , the supernatant was removed, the cells washed and then inoculated with pseudotypes, as described below.
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