Selected article for: "FBS fetal bovine serum and Hyclone 10 FBS fetal bovine serum"
Author: Qian, Shaoju; Gao, Zitong; Cao, Rui; Yang, Kang; Cui, Yijie; Li, Shaowen; Meng, Xianrong; He, Qigai; Li, Zili
Title: Transmissible Gastroenteritis Virus Infection Up-Regulates FcRn Expression via Nucleocapsid Protein and Secretion of TGF-ß in Porcine Intestinal Epithelial Cells
  • Document date: 2020_1_21
    • ID: 06qddkw0_5
    Snippet: IPEC-J2 cells donated by Xiaoping Li from Huazhong Agricultural University (Hubei Province, Wuhan, China) were cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone, United States) containing 10% fetal bovine serum (FBS; Gibco, United States) and 1% penicillin/streptomycin at 37 • C in a 5% CO 2 atmosphere. The isolated TGEV strain WH-1 (GenBank HQ462571) was propagated in IPEC-J2 cells. In our laboratory, AffiniPure rabbit anti-cytopla.....
    Document: IPEC-J2 cells donated by Xiaoping Li from Huazhong Agricultural University (Hubei Province, Wuhan, China) were cultured in Dulbecco's modified Eagle's medium (DMEM; Hyclone, United States) containing 10% fetal bovine serum (FBS; Gibco, United States) and 1% penicillin/streptomycin at 37 • C in a 5% CO 2 atmosphere. The isolated TGEV strain WH-1 (GenBank HQ462571) was propagated in IPEC-J2 cells. In our laboratory, AffiniPure rabbit anti-cytoplasmic tails of porcine FcRn (anti-FcRn-CT) polyclonal antibodies were prepared (Guo et al., 2016a) . Horseradish peroxidase (HRP)-conjugated goat anti-rabbit and goat anti-mouse IgG were purchased from Abclona (China). Monoclonal antibody (mAb) against GAPDH in mice was purchased from Abclona (China). Mouse mAbs against HA, IκBα, NF-κB, p65 and rabbit mAbs against phospho-NF-κB and p65 were obtained from Cell Signaling Technology (United States).

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