Selected article for: "acid detection and magnapure lc"
Author: van Rijn, Anneloes L.; van Boheemen, Sander; Sidorov, Igor; Carbo, Ellen C.; Pappas, Nikos; Mei, Hailiang; Feltkamp, Mariet; Aanerud, Marianne; Bakke, Per; Claas, Eric C. J.; Eagan, Tomas M.; Hiemstra, Pieter S.; Kroes, Aloys C. M.; de Vries, Jutte J. C.
Title: The respiratory virome and exacerbations in patients with chronic obstructive pulmonary disease
  • Document date: 2019_10_24
    • ID: 0rupk21u_16
    Snippet: Total nucleic acids (NA) were extracted directly from 200 μl clinical sample, using the Total Nucleic Acid extraction kit on the MagnaPure LC system (Roche Diagnostics, Almere, the Netherlands) with 100 μL output eluate. Nucleic acid amplification and detection by real-time PCR was performed on a BioRad CFX96 thermocycler, using primers, probes and conditions as described previously [19] . Cq values were normalized using a fixed baseline fluore.....
    Document: Total nucleic acids (NA) were extracted directly from 200 μl clinical sample, using the Total Nucleic Acid extraction kit on the MagnaPure LC system (Roche Diagnostics, Almere, the Netherlands) with 100 μL output eluate. Nucleic acid amplification and detection by real-time PCR was performed on a BioRad CFX96 thermocycler, using primers, probes and conditions as described previously [19] . Cq values were normalized using a fixed baseline fluorescence threshold.

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