Author: van Rijn, Anneloes L.; van Boheemen, Sander; Sidorov, Igor; Carbo, Ellen C.; Pappas, Nikos; Mei, Hailiang; Feltkamp, Mariet; Aanerud, Marianne; Bakke, Per; Claas, Eric C. J.; Eagan, Tomas M.; Hiemstra, Pieter S.; Kroes, Aloys C. M.; de Vries, Jutte J. C.
Title: The respiratory virome and exacerbations in patients with chronic obstructive pulmonary disease Document date: 2019_10_24
ID: 0rupk21u_16
Snippet: Total nucleic acids (NA) were extracted directly from 200 μl clinical sample, using the Total Nucleic Acid extraction kit on the MagnaPure LC system (Roche Diagnostics, Almere, the Netherlands) with 100 μL output eluate. Nucleic acid amplification and detection by real-time PCR was performed on a BioRad CFX96 thermocycler, using primers, probes and conditions as described previously [19] . Cq values were normalized using a fixed baseline fluore.....
Document: Total nucleic acids (NA) were extracted directly from 200 μl clinical sample, using the Total Nucleic Acid extraction kit on the MagnaPure LC system (Roche Diagnostics, Almere, the Netherlands) with 100 μL output eluate. Nucleic acid amplification and detection by real-time PCR was performed on a BioRad CFX96 thermocycler, using primers, probes and conditions as described previously [19] . Cq values were normalized using a fixed baseline fluorescence threshold.
Search related documents:
Co phrase search for related documents- baseline fluorescence threshold and real time: 1
- clinical sample and cq value: 1
- clinical sample and detection Nucleic acid amplification: 1
- clinical sample and extraction kit: 1, 2, 3, 4, 5, 6, 7
- clinical sample and μl clinical sample: 1, 2, 3, 4, 5
- clinical sample and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33
- clinical sample and Nucleic acid amplification: 1, 2, 3
- clinical sample and output eluate: 1
- clinical sample and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41
- clinical sample and total nucleic acid: 1, 2
- condition probe and real time: 1
- condition probe primer and real time: 1
- cq value and nucleic acid: 1
- cq value and real time: 1, 2, 3, 4, 5, 6, 7
- cq value and total nucleic acid: 1
- detection Nucleic acid amplification and extraction kit: 1
- detection Nucleic acid amplification and nucleic acid: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- detection Nucleic acid amplification and Nucleic acid amplification: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
- detection Nucleic acid amplification and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35
Co phrase search for related documents, hyperlinks ordered by date