Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Document date: 2017_1_12
ID: 0238bsxb_7
Snippet: MβCD and Mevastatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-IBV structural proteins S, N, and M, as well as the non-structural protein NSP5 and NSP12 antibodies were obtained via immunization of rabbits [33] . Mouse monoclonal antibodies against human tubulin and β-actin were purchased from Sigma-Aldrich along with anti-raft-resident marker caveolin-1 and GM1 antibodies. Fluorescein isothiocyanate (FITC)-conjugated antimous.....
Document: MβCD and Mevastatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-IBV structural proteins S, N, and M, as well as the non-structural protein NSP5 and NSP12 antibodies were obtained via immunization of rabbits [33] . Mouse monoclonal antibodies against human tubulin and β-actin were purchased from Sigma-Aldrich along with anti-raft-resident marker caveolin-1 and GM1 antibodies. Fluorescein isothiocyanate (FITC)-conjugated antimouse and anti-rabbit immunoglobulin G (IgG), as well as horseradish peroxidase (HRP)conjugated anti-human, anti-mouse, and anti-rabbit IgG were purchased from Dako (Glostrup, Denmark).
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