Author: Cooray, Samantha; Jin, Li; Best, Jennifer M
Title: The involvement of survival signaling pathways in rubella-virus induced apoptosis Document date: 2005_1_4
ID: 1i36lsj2_8
Snippet: The effect of RV infection on PI3K-Akt and Ras-Raf-MEK-ERK pathways was investigated by examining the expression and phosphorylation profiles of Akt, ERK1/2 and their downstream targets. Cell lysates from RV and mock infected RK13 cells were collected 12-96 hours post-infection (p.i.), separated by SDS-PAGE, and analyzed for total and phosphorylated Akt and ERK1/2 by Western blotting. Phosphorylated Akt and ERK1/2 could be detected in RVinfected .....
Document: The effect of RV infection on PI3K-Akt and Ras-Raf-MEK-ERK pathways was investigated by examining the expression and phosphorylation profiles of Akt, ERK1/2 and their downstream targets. Cell lysates from RV and mock infected RK13 cells were collected 12-96 hours post-infection (p.i.), separated by SDS-PAGE, and analyzed for total and phosphorylated Akt and ERK1/2 by Western blotting. Phosphorylated Akt and ERK1/2 could be detected in RVinfected cells from 48 hours p.i., and band intensity increased from 48-96 hours p.i. compared to total levels (Fig. 1A) . Phosphorylated Akt and ERK2 (but not ERK1) were detected in the mock-infected cells at 96 hours p.i. but not before, whereas total levels of Akt and ERK 1/2 were detectable at all time points (Fig. 1A) . Treatment of RV-infected cells with PI3K inhibitor LY294002 and MEK1/2 inhibitor U0126 completely inhibited activation of Akt and ERK1/2 respectively (data not shown).
Search related documents:
Co phrase search for related documents- Akt activation and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- band intensity and western blotting: 1, 2, 3, 4, 5, 6
- cell lysate and rv infection: 1
- cell lysate and time point: 1, 2
- cell lysate and total level: 1
- cell lysate and western blotting: 1, 2, 3, 4, 5, 6, 7
- downstream target and rv infection: 1, 2
- downstream target and western blotting: 1, 2, 3, 4, 5, 6
- phosphorylation expression and time point: 1
- phosphorylation expression and total level: 1
- phosphorylation expression and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- Ras Raf MEK ERK pathway and rv infection: 1
- Ras Raf MEK ERK pathway and western blotting: 1
- rv infection and time point: 1, 2, 3
- rv infection and western blotting: 1
- time point and total level: 1, 2, 3
- time point and western blotting: 1, 2, 3, 4, 5
- total level and western blotting: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date