Author: Hoffmann, Markus; González Hernández, Mariana; Berger, Elisabeth; Marzi, Andrea; Pöhlmann, Stefan
Title: The Glycoproteins of All Filovirus Species Use the Same Host Factors for Entry into Bat and Human Cells but Entry Efficiency Is Species Dependent Document date: 2016_2_22
ID: 146cwh6y_16
Snippet: For the transduction of cell lines (with or without prior treatment), the cells were seeded in 96-well plates (Thermo Scientific). At 24 h post seeding, the cell culture medium was removed and the cells were washed with PBS. Then, either undiluted or infectivity-normalized VSVpp were added (in quadruplicates) and the cells were further incubated for 1 h at 37°C and 5% CO 2 . Afterwards, the inoculum was removed and the cells were again washed wi.....
Document: For the transduction of cell lines (with or without prior treatment), the cells were seeded in 96-well plates (Thermo Scientific). At 24 h post seeding, the cell culture medium was removed and the cells were washed with PBS. Then, either undiluted or infectivity-normalized VSVpp were added (in quadruplicates) and the cells were further incubated for 1 h at 37°C and 5% CO 2 . Afterwards, the inoculum was removed and the cells were again washed with PBS and incubated with fresh culture medium for 16-18 h at 37°C and 5% CO 2 . To produce infectivitynormalized pseudotypes, the transduction efficiency (fLuc activity) was quantified in HEK-293T cells and the VSVpp were diluted in cell culture medium to yield comparable luciferase activity.
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