Author: Hoffmann, Markus; González Hernández, Mariana; Berger, Elisabeth; Marzi, Andrea; Pöhlmann, Stefan
Title: The Glycoproteins of All Filovirus Species Use the Same Host Factors for Entry into Bat and Human Cells but Entry Efficiency Is Species Dependent Document date: 2016_2_22
ID: 146cwh6y_16
Snippet: For the transduction of cell lines (with or without prior treatment), the cells were seeded in 96-well plates (Thermo Scientific). At 24 h post seeding, the cell culture medium was removed and the cells were washed with PBS. Then, either undiluted or infectivity-normalized VSVpp were added (in quadruplicates) and the cells were further incubated for 1 h at 37°C and 5% CO 2 . Afterwards, the inoculum was removed and the cells were again washed wi.....
Document: For the transduction of cell lines (with or without prior treatment), the cells were seeded in 96-well plates (Thermo Scientific). At 24 h post seeding, the cell culture medium was removed and the cells were washed with PBS. Then, either undiluted or infectivity-normalized VSVpp were added (in quadruplicates) and the cells were further incubated for 1 h at 37°C and 5% CO 2 . Afterwards, the inoculum was removed and the cells were again washed with PBS and incubated with fresh culture medium for 16-18 h at 37°C and 5% CO 2 . To produce infectivitynormalized pseudotypes, the transduction efficiency (fLuc activity) was quantified in HEK-293T cells and the VSVpp were diluted in cell culture medium to yield comparable luciferase activity.
Search related documents:
Co phrase search for related documents- cell culture and fluc activity: 1, 2
- cell culture and fluc activity transduction efficiency: 1, 2
- cell culture and luciferase activity: 1, 2, 3, 4, 5, 6
- cell culture and PBS wash: 1
- cell culture and prior treatment: 1, 2, 3, 4, 5
- cell culture medium and culture medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell culture medium and fluc activity: 1
- cell culture medium and fluc activity transduction efficiency: 1
- cell line and culture medium: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell line and fluc activity: 1
- cell line and luciferase activity: 1, 2, 3, 4, 5, 6, 7, 8, 9
- cell line and prior treatment: 1, 2, 3, 4, 5, 6, 7
- culture medium and fluc activity: 1
- culture medium and fluc activity transduction efficiency: 1
- culture medium and luciferase activity: 1, 2, 3
- fluc activity and luciferase activity: 1, 2, 3, 4, 5, 6, 7
- luciferase activity and PBS wash: 1
Co phrase search for related documents, hyperlinks ordered by date