Selected article for: "nucleic acid and PCR kit"
Author: Oude Munnink, Bas B; Canuti, Marta; Deijs, Martin; de Vries, Michel; Jebbink, Maarten F; Rebers, Sjoerd; Molenkamp, Richard; van Hemert, Formijn J; Chung, Kevin; Cotten, Matthew; Snijders, Fransje; Sol, Cees JA; van der Hoek, Lia
Title: Unexplained diarrhoea in HIV-1 infected individuals
  • Document date: 2014_1_13
    • ID: 0e9l19kp_19
    Snippet: VIDISCA and Roche Titanium-454 sequencing VIDISCA-454 was performed on 110 μl stool suspension as previously described by De Vries et al. [25] . VIDISCAamplified nucleic acid -each with its own multiplex identifier (MID) -was quantified with the Quant-it dsDNA HS Qubit kit (Invitrogen) and diluted to 10 6 copies/ml. All separate fractions were pooled and 4 pools from 14 different samples were used for a titration (DNA:beads ratio of 0.5:1, 1:1, .....
    Document: VIDISCA and Roche Titanium-454 sequencing VIDISCA-454 was performed on 110 μl stool suspension as previously described by De Vries et al. [25] . VIDISCAamplified nucleic acid -each with its own multiplex identifier (MID) -was quantified with the Quant-it dsDNA HS Qubit kit (Invitrogen) and diluted to 10 6 copies/ml. All separate fractions were pooled and 4 pools from 14 different samples were used for a titration (DNA:beads ratio of 0.5:1, 1:1, 2:1 and 4:1) in an emulsion PCR according to the suppliers' protocol (LIB-A SV emPCR kit). Sequencing was done on a 4 region GS FLX Titanium PicoTiterPlate (70x75) with the GS FLX Titanium XLR 70 Sequencing kit (Roche).

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