Author: Neufeldt, Christopher J.; Joyce, Michael A.; Van Buuren, Nicholas; Levin, Aviad; Kirkegaard, Karla; Gale Jr., Michael; Tyrrell, D. Lorne J.; Wozniak, Richard W.
Title: The Hepatitis C Virus-Induced Membranous Web and Associated Nuclear Transport Machinery Limit Access of Pattern Recognition Receptors to Viral Replication Sites Document date: 2016_2_10
ID: 1kuggdzj_56
Snippet: Immunofluorescence and western blotting were done as previously described [81] . Further details and a list of primary and secondary antibodies are provided in the extended Materials and Methods (S1 Text). Pearson's correlation coefficients were used to determine the total overlap between two fluorescent signals. These numbers were calculated as previously described using the Coloc2 plugin for ImageJ [82] . Percent overlap between positive-or neg.....
Document: Immunofluorescence and western blotting were done as previously described [81] . Further details and a list of primary and secondary antibodies are provided in the extended Materials and Methods (S1 Text). Pearson's correlation coefficients were used to determine the total overlap between two fluorescent signals. These numbers were calculated as previously described using the Coloc2 plugin for ImageJ [82] . Percent overlap between positive-or negative-strand probes and either RIG-I or ribosomes was determined using Manders colocalization coefficients. For graphs shown in the figures and percentages presented in the text, all imaging analysis was done on at least 10 different images with at least 15 individual cells. Quantification of the nuclear and cytoplasmic IRF-3 fluorescence levels in Fig 8 was
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