Author: Neufeldt, Christopher J.; Joyce, Michael A.; Van Buuren, Nicholas; Levin, Aviad; Kirkegaard, Karla; Gale Jr., Michael; Tyrrell, D. Lorne J.; Wozniak, Richard W.
Title: The Hepatitis C Virus-Induced Membranous Web and Associated Nuclear Transport Machinery Limit Access of Pattern Recognition Receptors to Viral Replication Sites Document date: 2016_2_10
ID: 1kuggdzj_60
Snippet: Subcellular fractions were serially diluted and added to Huh7.5 cells grown in optical 96 well plates. 2 days after infection, viral focus-forming units were determined by indirect immunofluorescence microscopy using antibodies specific to HCV core protein. The values for specific infectivity were calculated by dividing the number of Focus forming units by the total number of HCV RNA copies added to the cells (FFU/HCV RNA copy). The values for in.....
Document: Subcellular fractions were serially diluted and added to Huh7.5 cells grown in optical 96 well plates. 2 days after infection, viral focus-forming units were determined by indirect immunofluorescence microscopy using antibodies specific to HCV core protein. The values for specific infectivity were calculated by dividing the number of Focus forming units by the total number of HCV RNA copies added to the cells (FFU/HCV RNA copy). The values for infectious titer represent the number of focus forming units per ml in the culture medium harvested from each of the coinfected samples. The values for specific infectivity and infectious titer show an average over a count of 6 wells, and each experiment was repeated 3 times.
Search related documents:
Co phrase search for related documents- core protein and Subcellular fraction: 1
- culture medium and infectious titer: 1, 2, 3, 4, 5
- culture medium and total number: 1
Co phrase search for related documents, hyperlinks ordered by date