Author: David, Paul; Megger, Dominik A.; Kaiser, Tamara; Werner, Tanja; Liu, Jia; Chen, Lieping; Sitek, Barbara; Dittmer, Ulf; Zelinskyy, Gennadiy
Title: The PD-1/PD-L1 Pathway Affects the Expansion and Function of Cytotoxic CD8(+) T Cells During an Acute Retroviral Infection Document date: 2019_2_5
ID: 0ael8imp_35_1
Snippet: −/− and red for comparison with PD-L1 −/− ) are indicated (*p < 0.05, **p < 0.005). CFSE labeled spleen cells from naïve CD45.1 mice were loaded with FV peptide and were injected intravenously into naïve and 8 or 15 days infected mice. As controls similar number of CD45.1 spleen cells from naïve mice without peptide were co-injected into the same recipient mice. The spleen and bone marrow cells from recipient mice were isolated 2 h aft.....
Document: −/− and red for comparison with PD-L1 −/− ) are indicated (*p < 0.05, **p < 0.005). CFSE labeled spleen cells from naïve CD45.1 mice were loaded with FV peptide and were injected intravenously into naïve and 8 or 15 days infected mice. As controls similar number of CD45.1 spleen cells from naïve mice without peptide were co-injected into the same recipient mice. The spleen and bone marrow cells from recipient mice were isolated 2 h after injection and analyzed for numbers of CD45.1 + and CFSE fluorescence. The figure shows the percentage of eliminated FV peptide-loaded donor cells in spleen (E) and bone marrow (F). Each dot represents an individual mouse and the mean numbers and SD are indicated. Differences were analyzed by one-way ANOVA with a Tukey post-test. Statistically significant differences between the groups are indicated (*p < 0.05, **p < 0.005, ***p < 0.0005).
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