Author: Patton, John B.; Bennuru, Sasisekhar; Eberhard, Mark L.; Hess, Jessica A.; Torigian, April; Lustigman, Sara; Nutman, Thomas B.; Abraham, David
Title: Development of Onchocerca volvulus in humanized NSG mice and detection of parasite biomarkers in urine and serum Document date: 2018_12_12
ID: 0yh5k6jk_8
Snippet: Control and potential elimination of onchocerciasis has been significantly impeded by the limited number of available drugs and the development of resistance to those therapies [37, 38] . In addition, biomarkers to assess the infection status of treated individuals or macrofilaricidal activity are sorely lacking [6] [7] [8] . One of the critical barriers blocking drug and biomarker development has been the absence of suitable small animal hosts f.....
Document: Control and potential elimination of onchocerciasis has been significantly impeded by the limited number of available drugs and the development of resistance to those therapies [37, 38] . In addition, biomarkers to assess the infection status of treated individuals or macrofilaricidal activity are sorely lacking [6] [7] [8] . One of the critical barriers blocking drug and biomarker development has been the absence of suitable small animal hosts for experimentation. Hence, this study was focused on the development of a small animal model that would support the growth and maturation of O. volvulus and could serve to identify parasite specific biomarkers. To this end we tested multiple genetically defined mouse strains and xenografted (with human cells) immunodeficient mice to identify those microenvironments suitable for O. volvulus development. In so doing, we were able to develop convenient and tractable murine models that support the development of O. volvulus L3 into advanced larval stages. Moreover, we were able to use these O. volvulus-infected animals to identify parasite-derived biomarkers measurable in both urine and serum.
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