Selected article for: "cdna synthesis and Total RNA extraction"
Author: Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo-Yun; Li, Kui
Title: The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function
  • Document date: 2019_6_28
    • ID: 1nr0hggt_18
    Snippet: Extraction of total cellular RNA by TRIzol (Invitrogen), cDNA synthesis by reverse transcription, and quantitative PCR (qPCR) were implemented as described elsewhere [25, 41] . The following primers were used to detect ZIKV RNA (specifically recognizing the NS4B-coding region): ZIKV-4B-7260F, 5'-GCACTACATGTACTTGATC-3'; and ZIKV-4B-7367R, 5'-ACC ACTATTCCATCCACAAC-3'. Primers specific for human innate immune genes including TLR3, IFNB, IL29, RANTES.....
    Document: Extraction of total cellular RNA by TRIzol (Invitrogen), cDNA synthesis by reverse transcription, and quantitative PCR (qPCR) were implemented as described elsewhere [25, 41] . The following primers were used to detect ZIKV RNA (specifically recognizing the NS4B-coding region): ZIKV-4B-7260F, 5'-GCACTACATGTACTTGATC-3'; and ZIKV-4B-7367R, 5'-ACC ACTATTCCATCCACAAC-3'. Primers specific for human innate immune genes including TLR3, IFNB, IL29, RANTES and ISG56 have been described [34, 42] . The relative abundance of each target was normalized to that of 28S rRNA [34] . Copy numbers of ZIKV RNA were calculated based on standard curves generated using serially diluted pEF6-ZIKV-NS4B-V5His6 DNA that ranged from 10 4 to 10 7 copies/ml.

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