Author: David, Paul; Megger, Dominik A.; Kaiser, Tamara; Werner, Tanja; Liu, Jia; Chen, Lieping; Sitek, Barbara; Dittmer, Ulf; Zelinskyy, Gennadiy
Title: The PD-1/PD-L1 Pathway Affects the Expansion and Function of Cytotoxic CD8(+) T Cells During an Acute Retroviral Infection Document date: 2019_2_5
ID: 0ael8imp_15
Snippet: CD8 + T cells were positively isolated by magnetic activated cell sorting using 50 µl CD8a (Ly-2) microbeads (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions. Efficiency of cell separation was proven by flow cytometry (92% CD8 + T cells). Ten thousand purified cells were transferred into recipient WT or PD-L1 −/− mice by i.v. injection in 100 µl PBS......
Document: CD8 + T cells were positively isolated by magnetic activated cell sorting using 50 µl CD8a (Ly-2) microbeads (MACS, Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions. Efficiency of cell separation was proven by flow cytometry (92% CD8 + T cells). Ten thousand purified cells were transferred into recipient WT or PD-L1 −/− mice by i.v. injection in 100 µl PBS.
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