Author: Joshi, Shilvi; Chen, Lang; Winter, Michael B.; Lin, Yi-Lun; Yang, Yang; Shapovalova, Mariya; Smith, Paige M.; Liu, Chang; Li, Fang; LeBeau, Aaron M.
Title: The Rational Design of Therapeutic Peptides for Aminopeptidase N using a Substrate-Based Approach Document date: 2017_5_2
ID: 0pmo3opx_21
Snippet: APN expression and purification. Porcine APN ectodomain (residues 62-963) and human APN ecdomain (residues 66-967) were expressed and purified as previously described 31 . Briefly, APN ectodomains containing N-terminal honeybee-melittin signal peptide and C-terminal His 6 tag were expressed in insect cells, secreted to cell culture medium, and purified sequentially on Ni-NTA column and gel-filtration column......
Document: APN expression and purification. Porcine APN ectodomain (residues 62-963) and human APN ecdomain (residues 66-967) were expressed and purified as previously described 31 . Briefly, APN ectodomains containing N-terminal honeybee-melittin signal peptide and C-terminal His 6 tag were expressed in insect cells, secreted to cell culture medium, and purified sequentially on Ni-NTA column and gel-filtration column.
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