Selected article for: "amino acid and UniProt database"
Author: David, Paul; Megger, Dominik A.; Kaiser, Tamara; Werner, Tanja; Liu, Jia; Chen, Lieping; Sitek, Barbara; Dittmer, Ulf; Zelinskyy, Gennadiy
Title: The PD-1/PD-L1 Pathway Affects the Expansion and Function of Cytotoxic CD8(+) T Cells During an Acute Retroviral Infection
  • Document date: 2019_2_5
    • ID: 0ael8imp_23
    Snippet: Cells were lysed by sonication (6 10-s pulses on ice) in sample buffer (50 mM NH4HCO3; 0.1% Rapigest). Protein isolation, digestion, amino acid analysis, and liquid chromatographytandem mass spectrometry (LC-MS/MS) analysis were performed by following previously described procedures (25) . Progenesis QI for proteomics (v. 2.0.5387.52102; Nonlinear Dynamics, Newcastle upon Tyne, UK) and Proteome Discoverer (v. 1.4; Thermo Scientific, Bremen, Germa.....
    Document: Cells were lysed by sonication (6 10-s pulses on ice) in sample buffer (50 mM NH4HCO3; 0.1% Rapigest). Protein isolation, digestion, amino acid analysis, and liquid chromatographytandem mass spectrometry (LC-MS/MS) analysis were performed by following previously described procedures (25) . Progenesis QI for proteomics (v. 2.0.5387.52102; Nonlinear Dynamics, Newcastle upon Tyne, UK) and Proteome Discoverer (v. 1.4; Thermo Scientific, Bremen, Germany) software packages were used for protein quantification and identification, respectively. For protein identification, LC-MS/MS runs were searched against the UniProt-SwissProt database (Release 2014_10; v. 2.5; 546,790 sequences) with taxonomy restriction to mus musculus. Further search parameters matched previously reported ones (25) . Non-unique peptides associated to more than one protein accession were not used for quantification. Differentially expressed proteins (p < 0.05) were functionally annotated using the Database for Annotation, Visualization, and Integrated Discovery (DAVID, ver. 6.8) (26, 27) .

    Search related documents:
    Co phrase search for related documents
    • amino acid analysis and mass spectrometry: 1, 2, 3
    • amino acid analysis and musculus mus: 1
    • amino acid analysis and protein accession: 1
    • amino acid analysis and protein identification: 1
    • amino acid and LC MS MS analysis: 1, 2, 3, 4, 5, 6, 7
    • amino acid and LC MS MS run: 1
    • amino acid and mass spectrometry: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
    • amino acid and musculus mus: 1, 2, 3, 4
    • amino acid and previously describe: 1, 2, 3
    • amino acid and protein accession: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • amino acid and protein identification: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • amino acid and protein isolation: 1, 2
    • amino acid and protein quantification: 1
    • amino acid and software package: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • identification protein quantification and mass spectrometry: 1, 2, 3, 4
    • identification protein quantification and protein identification: 1, 2, 3, 4, 5, 6, 7
    • identification protein quantification and protein quantification: 1, 2, 3, 4, 5, 6, 7, 8
    • LC MS MS analysis and mass spectrometry: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • LC MS MS analysis and protein identification: 1