The Screening Research of NF-?B Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS

Lu, Yujie; Liu, Wenjuan; Zhang, Man; Deng, Yanfang; Jiang, Min; Bai, Gang

To identify the anti-inflammatory components in MC, we performed a dual-luciferase reporter assay integrated UPLC-Q/TOF-MS. Representative chemical components of MC and the total ion current chromatograms in positive and negative ESI modes, respectively, are shown in Figures 2(a) and 2(b) . In total, seven fractions showed potential NF-B inhibitor activity (Figure 2(c) ). We analyzed the seven fractions, and compounds they contain were identified by exact molecular weights and diagnostic fragment ions (Figure 2(d) ). The detailed results and MS/MS information were shown in Table 1 . The seven potential NF-B inhibitors could be classified into two types according to their chemical structures: monoterpenes (oxypaeoniflorin, paeoniflorin, (1) gallic acid (2) galloylpaeoniflorin, benzoyloxypaeoniflorin, mudanpioside C) and phenolic acids (gallic acid, paeonol). Among them, the NF-B inhibitory activities of galloylpaeoniflorin, benzoyloxypaeoniflorin, and mudanpioside C are first reported here. We then tested and verified the anti-inflammatory activity of these monomers.