Selected article for: "amplicon vector and vector immunization"
Author: Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.
Title: Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector
  • Document date: 2017_2_16
    • ID: 09hmet7r_50
    Snippet: Helper virus-free HSV-1 amplicon vector stocks were prepared as previously described [16, 26] . Briefly, Vero 2-2 cells were co-transfected with amplicon plasmid DNA, the fHSV∆pac∆ICP27 BAC DNA, and plasmid pEBHICP27 using Lipofectamine LTX and Plus Reagent (Thermo Fisher Scientific). After 72 h, cells were scraped into the medium, freeze/thawed, sonicated, and the cell debris was removed by centrifugation. For immunization of mice, vector st.....
    Document: Helper virus-free HSV-1 amplicon vector stocks were prepared as previously described [16, 26] . Briefly, Vero 2-2 cells were co-transfected with amplicon plasmid DNA, the fHSV∆pac∆ICP27 BAC DNA, and plasmid pEBHICP27 using Lipofectamine LTX and Plus Reagent (Thermo Fisher Scientific). After 72 h, cells were scraped into the medium, freeze/thawed, sonicated, and the cell debris was removed by centrifugation. For immunization of mice, vector stocks were further purified and concentrated by centrifugation over a 25% sucrose cushion. For titration, Vero 2-2 cells were infected with the amplicon vectors, and after 24 h stained for immune fluorescence using the appropriate antibodies and fluorescent cells were counted using an inverted fluorescence microscope (Axio Observer inverted microscope, Zeiss AG, Oberkochen, Germany). The titers were determined as TU/mL.

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