The Screening Research of NF-?B Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS

Lu, Yujie; Liu, Wenjuan; Zhang, Man; Deng, Yanfang; Jiang, Min; Bai, Gang

Culture. The HEK 293 cells were purchased from American Type Culture Collection (Rockville, MD, USA) and were cultured in DMEM high glucose with 10% FBS, 100 IU/mL penicillin, and 100 mg/mL streptomycin at 37 ∘ C and 5% CO 2 in a thermostatic incubator. BEAS-2B, derived from human bronchial epithelial cells, was also purchased from the American Type Culture Collection (Rockville, MD, USA). Their culture conditions were the same as those of HEK 293 cells, except for DMEM/F-12 (HAM) 1:1 instead of DMEM high glucose. group, and MC-L (0.01 mg/mL) group. All the cells except for cells in the control group were stimulated with tumor necrosis factor-(TNF-, 20 ng/mL) and simultaneously given with drugs for 6 h. Among them, Dex was used as a positive control and could decrease the expression of NF-B in HEK 293 cells. Relative content of NF-B in each group was represented as relative light unit (RLU) ratio following the manufacturer's instructions.