Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_104
Snippet: Movie S5. Live cell imaging reveals that N-BAR generates tubules with longer lifetime compared to Amph-FL and N-BAR-NfM CTD. Movie shows tubules in live RPE cells expressing N-BAR (left), Amph-FL (middle), or N-BAR-NfM CTD (right), imaged by TIRF microscopy at 37 °C. The cells are all at similar protein expression level, and were imaged using identical settings. The N-BAR-expressing cell shows a greater number of tubules that also persist longer.....
Document: Movie S5. Live cell imaging reveals that N-BAR generates tubules with longer lifetime compared to Amph-FL and N-BAR-NfM CTD. Movie shows tubules in live RPE cells expressing N-BAR (left), Amph-FL (middle), or N-BAR-NfM CTD (right), imaged by TIRF microscopy at 37 °C. The cells are all at similar protein expression level, and were imaged using identical settings. The N-BAR-expressing cell shows a greater number of tubules that also persist longer compared to Amph-FL and N-BAR-NfM CTD. See Fig. 4F for quantification. The frames are 2 s apart, 120 frames total. The video plays as 10 frames per second.
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