Author: Lu, Yujie; Liu, Wenjuan; Zhang, Man; Deng, Yanfang; Jiang, Min; Bai, Gang
Title: The Screening Research of NF-?B Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS Document date: 2019_3_3
ID: 1ahxau9k_10
Snippet: The freeze-dried powder of MC extract was accurately weighed and ultrasonic dissolved in methanol (1 mg/mL). After column separation, the 10% fractions were transported to the Q-TOF/MS system for components identification and the 90% fractions were collected every 0.5 min into a deep 96-well plate and vacuum dried at 56 ∘ C. The residues were dissolved in DMEM (50 L) for dual-luciferase assay. The operation process was the same as above. Screen.....
Document: The freeze-dried powder of MC extract was accurately weighed and ultrasonic dissolved in methanol (1 mg/mL). After column separation, the 10% fractions were transported to the Q-TOF/MS system for components identification and the 90% fractions were collected every 0.5 min into a deep 96-well plate and vacuum dried at 56 ∘ C. The residues were dissolved in DMEM (50 L) for dual-luciferase assay. The operation process was the same as above. Screening out fractions that could reduce the level of NF-B was performed for further structural analysis.
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