Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_106
Snippet: Movie S7. The I-BAR-AP180 CTD chimera drives frustrated membrane fluctuations. GUV composition: 79.5 mol% DOPC, 5 mol% PtdIns(4,5)P 2 , 15 mol% DOPS, 0.5 mol% Oregon Green 488-DHPE. GUVs were mixed with 10 µM I-BAR-AP180 CTD and imaged by confocal microscopy. Fluorescence signal comes from Atto594-labeled protein. The frames are approximately 500 ms apart. The video plays at 5 frames per second......
Document: Movie S7. The I-BAR-AP180 CTD chimera drives frustrated membrane fluctuations. GUV composition: 79.5 mol% DOPC, 5 mol% PtdIns(4,5)P 2 , 15 mol% DOPS, 0.5 mol% Oregon Green 488-DHPE. GUVs were mixed with 10 µM I-BAR-AP180 CTD and imaged by confocal microscopy. Fluorescence signal comes from Atto594-labeled protein. The frames are approximately 500 ms apart. The video plays at 5 frames per second.
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