Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_16
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/276147 doi: bioRxiv preprint Figure 3 . The disordered domain of amphiphysin alone drives membrane fission, but the N-BAR scaffold substantially enhances fission efficiency. Membrane composition in Amph CTD ∆SH3 tethered vesicle experiments: 76 mol% DOPC, 20 mol% DOGS-NTA-Ni, 2 mol% Oregon Green 488-DHPE, 2 mol% DP-EG10-biotin. I.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/276147 doi: bioRxiv preprint Figure 3 . The disordered domain of amphiphysin alone drives membrane fission, but the N-BAR scaffold substantially enhances fission efficiency. Membrane composition in Amph CTD ∆SH3 tethered vesicle experiments: 76 mol% DOPC, 20 mol% DOGS-NTA-Ni, 2 mol% Oregon Green 488-DHPE, 2 mol% DP-EG10-biotin. In tethered vesicle experiments with N-BAR-epsin CTD, DOGS-NTA-Ni was replaced with 5 mol% PtdIns(4,5)P 2 and 15 mol% DOPS. SUPER template membrane composition: 79 mol% DOPC, 5 mol% PtdIns(4,5)P 2 , 15 mol% DOPS, 1 mol% Texas Red-DHPE.
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