The Screening Research of NF-?B Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS

Lu, Yujie; Liu, Wenjuan; Zhang, Man; Deng, Yanfang; Jiang, Min; Bai, Gang

In conclusion, MC showed significant efficacy in inhibiting NF-B activation, and seven bioactive components were screened by a dual-luciferase reporter assay integrated UPLC-Q/TOF-MS. According to their structural characteristics, the potential NF-B inhibitors could be categorized into two types: monoterpenes (oxypaeoniflorin, paeoniflorin, galloylpaeoniflorin, benzoyloxypaeoniflorin, mudanpioside C) and phenolic acids (gallic acid, paeonol). Thereinto, galloylpaeoniflorin, benzoyloxypaeoniflorin, and mudanpioside C were first reported to the effects of inhibiting the activation of NF-B. The present study demonstrates that MC contains a variety of structurally diverse anti-inflammatory active ingredients, acting on different targets, which provides a basis for the discovery of novel anti-inflammatory drugs with fewer side effects. And this article may provide a useful reference for improving the quality standards of MC in the future. Additionally, these experimental results also showed that the bioactivity-integrated UPLC-Q/TOF which contain both chemical and bioactive details is suitable for screening active ingredients from natural medicines.