Selected article for: "amplification efficiency and reference gene"

Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site
  • Document date: 2015_3_25
  • ID: 10p3mth2_12
    Snippet: For the fluorophore assay, whole cell lysates were transferred to black 96-well plates (Greiner Bio-One) and relative fluorescence units were measured using a BMG POLARstar OPTI-MA (BMG Labtech). EGFP (5´reporter) and DsRed.T4 (3´reporter) fluorescence was detected using 485 nm excitation/520 nm emission and 525 nm excitation/600 nm emission spectra, respectively. Relative frameshift efficiency was calculated as above. . Expression levels were .....
    Document: For the fluorophore assay, whole cell lysates were transferred to black 96-well plates (Greiner Bio-One) and relative fluorescence units were measured using a BMG POLARstar OPTI-MA (BMG Labtech). EGFP (5´reporter) and DsRed.T4 (3´reporter) fluorescence was detected using 485 nm excitation/520 nm emission and 525 nm excitation/600 nm emission spectra, respectively. Relative frameshift efficiency was calculated as above. . Expression levels were calculated using the comparative C T method of relative quantification, after confirming that amplification efficiency of the target gene was within 5% of the reference gene efficiency.

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