Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site Document date: 2015_3_25
ID: 10p3mth2_12
Snippet: For the fluorophore assay, whole cell lysates were transferred to black 96-well plates (Greiner Bio-One) and relative fluorescence units were measured using a BMG POLARstar OPTI-MA (BMG Labtech). EGFP (5´reporter) and DsRed.T4 (3´reporter) fluorescence was detected using 485 nm excitation/520 nm emission and 525 nm excitation/600 nm emission spectra, respectively. Relative frameshift efficiency was calculated as above. . Expression levels were .....
Document: For the fluorophore assay, whole cell lysates were transferred to black 96-well plates (Greiner Bio-One) and relative fluorescence units were measured using a BMG POLARstar OPTI-MA (BMG Labtech). EGFP (5´reporter) and DsRed.T4 (3´reporter) fluorescence was detected using 485 nm excitation/520 nm emission and 525 nm excitation/600 nm emission spectra, respectively. Relative frameshift efficiency was calculated as above. . Expression levels were calculated using the comparative C T method of relative quantification, after confirming that amplification efficiency of the target gene was within 5% of the reference gene efficiency.
Search related documents:
Co phrase search for related documents- cell lysate and frameshift efficiency: 1, 2
- cell lysate and nm emission: 1
- cell lysate and reference gene: 1
- cell lysate and relative frameshift efficiency: 1, 2
- expression level and nm emission: 1
- expression level and reference gene: 1, 2, 3, 4
- expression level and relative quantification: 1, 2
- expression level and target gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- frameshift efficiency and reference gene: 1
- frameshift efficiency and relative frameshift efficiency: 1, 2, 3, 4
- frameshift efficiency and target gene: 1
- nm emission and relative fluorescence: 1, 2
- nm emission and relative fluorescence unit: 1
- reference gene and relative frameshift efficiency: 1
- reference gene and relative quantification: 1, 2, 3
- reference gene and target gene: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
- reference gene efficiency and target gene: 1
- relative frameshift efficiency and target gene: 1
- relative quantification and target gene: 1, 2, 3, 4, 5
Co phrase search for related documents, hyperlinks ordered by date