Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains Document date: 2018_3_4
ID: drqseaaa_67
Snippet: for 10-15 min to dissolve the PEG. Acetic acid was added to a concentration of 1%, and 50 µL of the reactive mixture was dropped onto a dry, ultraclean coverslip. Another dry, ultraclean coverslip was sandwiched on top, and the slides were incubated at 70 °C for 30-60 min. The slides were separated, washed in ultrapure water, and stored dry for later use. Imaging wells were made by placing silicone gaskets onto the glass and hydrating in 20 mM .....
Document: for 10-15 min to dissolve the PEG. Acetic acid was added to a concentration of 1%, and 50 µL of the reactive mixture was dropped onto a dry, ultraclean coverslip. Another dry, ultraclean coverslip was sandwiched on top, and the slides were incubated at 70 °C for 30-60 min. The slides were separated, washed in ultrapure water, and stored dry for later use. Imaging wells were made by placing silicone gaskets onto the glass and hydrating in 20 mM MOPS pH 7.35, 150 mM NaCl buffer. Neutravidin was added to the well at a final concentration of 0.2 mg mL -1 , incubated for 10 min, and the well was washed repeatedly with MOPS buffer before adding vesicles.
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