Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site Document date: 2015_3_25
ID: 10p3mth2_34
Snippet: We utilised the human tRNA Ser anticodon that had been previously modified to produce variants that would recognise UAA, UAG and UGA [41] . To first test their specificity in the cultured cells, readthrough of the efficient termination signals UAAAAG, UAGAAG and UGAAAG were measured in HEK293T cells with the cognate suppressor, the non-cognate suppressors and parent tRNA Ser as the negative control. All test stop signals allowed only < 0.1% readt.....
Document: We utilised the human tRNA Ser anticodon that had been previously modified to produce variants that would recognise UAA, UAG and UGA [41] . To first test their specificity in the cultured cells, readthrough of the efficient termination signals UAAAAG, UAGAAG and UGAAAG were measured in HEK293T cells with the cognate suppressor, the non-cognate suppressors and parent tRNA Ser as the negative control. All test stop signals allowed only < 0.1% readthrough with co-expression of either the negative control tRNA Ser or the The Codon following the Slippery Sequence Supports HIV Frameshifting non-cognate suppressors. Cognate suppressors increased readthrough to 9-16% at their respective termination signals 48 h after transfection (Fig. 6A) .
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