Author: Shapira, Assaf; Benhar, Itai
Title: Toxin-Based Therapeutic Approaches Document date: 2010_10_28
ID: 00cf294x_60
Snippet: An in vitro study in reticulocyte lysates indicated that the ten N terminal amino acids that confer the toxin with short in vitro half life were also the most destabilizing ones in vivo. Moreover, cytotoxicity assays in Vero cells showed a clear correlation between the in vivo stability and the cytotoxicity of the proteins, although the difference in cytotoxicity between the wild-type DT-A fragment and the least stable mutant was only ~20-fold in.....
Document: An in vitro study in reticulocyte lysates indicated that the ten N terminal amino acids that confer the toxin with short in vitro half life were also the most destabilizing ones in vivo. Moreover, cytotoxicity assays in Vero cells showed a clear correlation between the in vivo stability and the cytotoxicity of the proteins, although the difference in cytotoxicity between the wild-type DT-A fragment and the least stable mutant was only ~20-fold in compared to ~100-fold difference in intracellular half-life [512] .
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