Author: Jemielity, Stephanie; Wang, Jinyize J.; Chan, Ying Kai; Ahmed, Asim A.; Li, Wenhui; Monahan, Sheena; Bu, Xia; Farzan, Michael; Freeman, Gordon J.; Umetsu, Dale T.; DeKruyff, Rosemarie H.; Choe, Hyeryun
Title: TIM-family Proteins Promote Infection of Multiple Enveloped Viruses through Virion-associated Phosphatidylserine Document date: 2013_3_28
ID: 0fais1pz_23
Snippet: MLVgag-GFP pseudovirions were produced similarly as described for the non-fluorescent pseudoviruses, except that 25% of the MLV gag-pol plasmid DNA was replaced with plasmid DNA encoding a MLV gag-GFP fusion protein [48] , a gift from Walther Mothes at Yale University School of Medicine. Also, pQCXIX-eGFP was replaced with the same vector encoding a nonfluorescent protein. Virus supernatants were harvested at 34-36 h post-transfection, 0.45 mm-fi.....
Document: MLVgag-GFP pseudovirions were produced similarly as described for the non-fluorescent pseudoviruses, except that 25% of the MLV gag-pol plasmid DNA was replaced with plasmid DNA encoding a MLV gag-GFP fusion protein [48] , a gift from Walther Mothes at Yale University School of Medicine. Also, pQCXIX-eGFP was replaced with the same vector encoding a nonfluorescent protein. Virus supernatants were harvested at 34-36 h post-transfection, 0.45 mm-filtered, purified by ultracentrifugation (SW40Ti rotor, 70'000 g for 2 h at 10uC) and resuspended in a small volume of DMEM containing 10% FBS.
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