Toll-like receptors, chemokine receptors and death receptor ligands responses in SARS coronavirus infected human monocyte derived dendritic cells

Law, Helen KW; Cheung, Chung Yan; Sia, Sin Fun; Chan, Yuk On; Peiris, JS Malik; Lau, Yu Lung

TLRs are expressed on many cell types and their expression on different DC subsets have been characterised. Visintin et al. [22] measured TLR expression on immature human DCs by monoclonal antibodies binding, but the expression was very low, corresponding to a few hundred molecules or less in immature DCs. Subsequent studies of TLRs expressions were based on comparing RNA expression quantitatively and some reports for immature DCs have been controversial. Our detection of TLR-1 to TLR-9 expressions in mock infected adult DCs were consistent with that reported by Jarrossay et al. [10] and the low level of TLR-10 was similar to that reported by Kadowaki et al. [11] .