Selected article for: "cell remove and culture supernatant"
Author: Law, Helen KW; Cheung, Chung Yan; Sia, Sin Fun; Chan, Yuk On; Peiris, JS Malik; Lau, Yu Lung
Title: Toll-like receptors, chemokine receptors and death receptor ligands responses in SARS coronavirus infected human monocyte derived dendritic cells
  • Document date: 2009_6_8
    • ID: 19noki6p_36
    Snippet: Laboratory procedures involving live viruses was performed in biosafety level-3 containment. SARS-CoV, strain HKU-39849 [1] was cultured in fetal rhesus kidney-4 (FRhK-4) cells. The cell culture supernatant was harvested, centrifuged to remove cell fragments, aliquoted and kept frozen at -70°C. SARS-CoV titre of the stock virus was determined by infection of FRhK-4 cells. Cytopathic changes on FRhK-4 cells was monitored every day up to 4 days an.....
    Document: Laboratory procedures involving live viruses was performed in biosafety level-3 containment. SARS-CoV, strain HKU-39849 [1] was cultured in fetal rhesus kidney-4 (FRhK-4) cells. The cell culture supernatant was harvested, centrifuged to remove cell fragments, aliquoted and kept frozen at -70°C. SARS-CoV titre of the stock virus was determined by infection of FRhK-4 cells. Cytopathic changes on FRhK-4 cells was monitored every day up to 4 days and virus titre expressed as tissue culture infective dose (TCID 50 ).

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