Author: Holman, Devin B.; Timsit, Edouard; Amat, Samat; Abbott, D. Wade; Buret, Andre G.; Alexander, Trevor W.
Title: The nasopharyngeal microbiota of beef cattle before and after transport to a feedlot Document date: 2017_3_22
ID: 1nni3vhm_7
Snippet: Upon arrival at the feedlot, the heifers were not mixed with cattle from other sources and were fed alfalfabarley silage mixed diets similar to the ones at the disease-free farm. Nasopharyngeal samples were collected from each calf in the study on days 0 (at the disease-free farm prior to shipment), 2, 7, and 14 according to Timsit et al. [3] . Prior to sampling, the nostril was wiped clean with 70% ethanol. Extended guarded swabs (27 cm) with a .....
Document: Upon arrival at the feedlot, the heifers were not mixed with cattle from other sources and were fed alfalfabarley silage mixed diets similar to the ones at the disease-free farm. Nasopharyngeal samples were collected from each calf in the study on days 0 (at the disease-free farm prior to shipment), 2, 7, and 14 according to Timsit et al. [3] . Prior to sampling, the nostril was wiped clean with 70% ethanol. Extended guarded swabs (27 cm) with a rayon bud (MW 124, Medical Wire & Equipment, Corsham, England) were used for sampling (Additional file 1: Fig. S1 ) and swabs were transported to the lab on ice for processing, within one hour of collection. Animals used in this study were cared for according to the guidelines set by the Canadian Council on Animal Care [13] and all experimental procedures involving cattle were approved by the Animal Care Committee of the Lethbridge Research Centre.
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