Author: Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.
Title: Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector Document date: 2017_2_16
ID: 09hmet7r_11
Snippet: Synthesis of the RV proteins mediated by the synthetic HSV-1 amplicon vectors sWa[VP2/6/7] and sWa[VP2/6/7_V5] was confirmed by Western blot analysis of total cell lysates following transduction of cells. All three RV proteins VP2, VP6 and VP7 were synthesized in human and non-human cells shown in Figure 1B for the human hepatocellular cells HepG2. Furthermore, the V5 tagged VP7 (VP7_V5) was detected using the V5 antibody in cells transduced with.....
Document: Synthesis of the RV proteins mediated by the synthetic HSV-1 amplicon vectors sWa[VP2/6/7] and sWa[VP2/6/7_V5] was confirmed by Western blot analysis of total cell lysates following transduction of cells. All three RV proteins VP2, VP6 and VP7 were synthesized in human and non-human cells shown in Figure 1B for the human hepatocellular cells HepG2. Furthermore, the V5 tagged VP7 (VP7_V5) was detected using the V5 antibody in cells transduced with sWa[VP2/6/7_V5] ( Figure 1B , middle panel).
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