Author: Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.
Title: Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector Document date: 2017_2_16
ID: 09hmet7r_58
Snippet: For immune electron microscopy, samples were adsorbed to carbon-coated parlodion films mounted on 300 mesh/inch copper grids (EMS) for 10 min, blocked with PBS containing 0.1% BSA (PBS-BSA/0.1%) for 10 min, incubated with the polyclonal rabbit anti-RV serum (strain RF, provided by Didier Poncet, CNRS/INRA, Gif-sur-Yvette, France) at a dilution of 1:1000 PBS-BSA/0.1% for 1 h, washed several times with PBS-BSA/0.1%, incubated with goat anti-rabbit .....
Document: For immune electron microscopy, samples were adsorbed to carbon-coated parlodion films mounted on 300 mesh/inch copper grids (EMS) for 10 min, blocked with PBS containing 0.1% BSA (PBS-BSA/0.1%) for 10 min, incubated with the polyclonal rabbit anti-RV serum (strain RF, provided by Didier Poncet, CNRS/INRA, Gif-sur-Yvette, France) at a dilution of 1:1000 PBS-BSA/0.1% for 1 h, washed several times with PBS-BSA/0.1%, incubated with goat anti-rabbit IgG coupled to 12 nm colloidal gold particles (Jackson ImmunoResearch, West Grove, PA, USA), washed several times with PBS and H 2 O, and stained with 2% PTA, pH 7.0 (Aldrich) for 1 min. Specimens were analyzed in a transmission electron microscope (CM12, Philips) equipped with a CCD camera (Ultrascan 1000, Gatan) at an acceleration voltage of 100 keV.
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