Selected article for: "µg ml and IgG detection"
Author: Meier, Anita F.; Suter, Mark; Schraner, Elisabeth M.; Humbel, Bruno M.; Tobler, Kurt; Ackermann, Mathias; Laimbacher, Andrea S.
Title: Transfer of Anti-Rotavirus Antibodies during Pregnancy and in Milk Following Maternal Vaccination with a Herpes Simplex Virus Type-1 Amplicon Vector
  • Document date: 2017_2_16
    • ID: 09hmet7r_71
    Snippet: For the evaluation of IgG isotypes (IgG1, IgG2a and IgG2b) standardized biotinylated isotype-specific monoclonal antibodies (IgG1: RMG1-1, 0.1 µg/mL, IgG2a: RMG2a-62, 0.2 µg/mL, IgG2b: RMG2b-1, 1.6 µg/mL, all from BioLegend, San Diego, CA, USA) and for detection HRP-conjugated streptavidin (1:2000, BioLegend, San Diego, CA, USA) were used. For the standardization IgG1; IgG2a; IgG2b monoclonal immunoglobulins were first coated in identical conc.....
    Document: For the evaluation of IgG isotypes (IgG1, IgG2a and IgG2b) standardized biotinylated isotype-specific monoclonal antibodies (IgG1: RMG1-1, 0.1 µg/mL, IgG2a: RMG2a-62, 0.2 µg/mL, IgG2b: RMG2b-1, 1.6 µg/mL, all from BioLegend, San Diego, CA, USA) and for detection HRP-conjugated streptavidin (1:2000, BioLegend, San Diego, CA, USA) were used. For the standardization IgG1; IgG2a; IgG2b monoclonal immunoglobulins were first coated in identical concentrations directly to ELISA plates as antigen, followed by various dilutions of the detection system and appropriate concentrations of the antibodies (given above) selected. Serum samples of dams and their pups (diluted 1:1000) were reanalyzed with the antigen coated as above but the IgG isotypes were detected with the appropriate standardized detection system.

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