Author: Qian, Shaoju; Gao, Zitong; Cao, Rui; Yang, Kang; Cui, Yijie; Li, Shaowen; Meng, Xianrong; He, Qigai; Li, Zili
Title: Transmissible Gastroenteritis Virus Infection Up-Regulates FcRn Expression via Nucleocapsid Protein and Secretion of TGF-ß in Porcine Intestinal Epithelial Cells Document date: 2020_1_21
ID: 06qddkw0_11
Snippet: The samples were harvested with lysis buffer (4% sodium dodecyl sulfate, 3% dithiothreitol, 65 mM Tris-HCl [pH 6.8], and 30% glycerol). Proteins were separated via SDS-PAGE and then electrotransferred onto a polyvinylidene difluoride membrane (Bio-Rad, United States). Western blot analysis was performed using the indicated antibodies following the procedure as described previously (Guo et al., 2016b) . IPEC-J2 cells grown in a six-well plate were.....
Document: The samples were harvested with lysis buffer (4% sodium dodecyl sulfate, 3% dithiothreitol, 65 mM Tris-HCl [pH 6.8], and 30% glycerol). Proteins were separated via SDS-PAGE and then electrotransferred onto a polyvinylidene difluoride membrane (Bio-Rad, United States). Western blot analysis was performed using the indicated antibodies following the procedure as described previously (Guo et al., 2016b) . IPEC-J2 cells grown in a six-well plate were infected with TGEV, the supernatant of IPEC-J2 cells was harvested, and IL-1β, IL-6, IL-8, TGF-β, and TNF-α protein levels were measured with a Quantibody Porcine Cytokine Array 1 (Ray Biotech, Norcross, GA, United States) according to the manufacturer's instructions.
Search related documents:
Co phrase search for related documents- blot analysis and protein level: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22
- blot analysis and TNF α: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- blot analysis and western blot analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- difluoride membrane and western blot analysis: 1
- protein level and TNF α: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- protein level and TNF α protein level: 1, 2
- protein level and western blot analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- TGEV infect and TNF α: 1
- TNF α and western blot analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date