Selected article for: "amino terminal and Flag epitope"

Author: Shapira, Assaf; Benhar, Itai
Title: Toxin-Based Therapeutic Approaches
  • Document date: 2010_10_28
  • ID: 00cf294x_59
    Snippet: In a comprehensive study by Falnes et al [512] , the in vivo stability and cytotoxicity of diphtheria toxin-based polypeptides, artificially modified to initiate with short sequences derived from the FLAG peptide epitope differing only in their N-terminal amino acids, were assessed. According to their findings, when the first N terminal amino acid of the modified toxins were Phe, Tyr, Trp, Asp, Asn, Glu, Gln, Lys, Arg or His, the proteins were hi.....
    Document: In a comprehensive study by Falnes et al [512] , the in vivo stability and cytotoxicity of diphtheria toxin-based polypeptides, artificially modified to initiate with short sequences derived from the FLAG peptide epitope differing only in their N-terminal amino acids, were assessed. According to their findings, when the first N terminal amino acid of the modified toxins were Phe, Tyr, Trp, Asp, Asn, Glu, Gln, Lys, Arg or His, the proteins were highly unstable, with half-lives in the range 0.2-1.6 hours, while the toxins initiated with either of the remaining amino acids were considerably more stable, with half-lives ranging from 3 to >12 hours.

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