Author: Qing, Enya; Hantak, Michael; Perlman, Stanley; Gallagher, Tom
Title: Distinct Roles for Sialoside and Protein Receptors in Coronavirus Infection Document date: 2020_2_11
ID: 1mowsbjy_26
Snippet: Pseudoviruses. Pseudovirus particles (pps) were constructed from a vesicular stomatitis virus (VSV) platform as described in reference 87. Briefly, HEK293T cells were transfected with viral glycoprotein expression plasmids, including pHEF-VSV G-Indiana (BEI Resources), pcDNA3.1-HA5-QH, and pcDNA3.1-PR8 NA1 (obtained from Lijun Rong, University of Illinois-Chicago). Twenty-four hours later, the cells were inoculated for 2 h with VSVdeltaG/Junin GP.....
Document: Pseudoviruses. Pseudovirus particles (pps) were constructed from a vesicular stomatitis virus (VSV) platform as described in reference 87. Briefly, HEK293T cells were transfected with viral glycoprotein expression plasmids, including pHEF-VSV G-Indiana (BEI Resources), pcDNA3.1-HA5-QH, and pcDNA3.1-PR8 NA1 (obtained from Lijun Rong, University of Illinois-Chicago). Twenty-four hours later, the cells were inoculated for 2 h with VSVdeltaG/Junin GP-luciferase (87, 88) . The cells were rinsed twice with FBS-free DMEM medium and replenished with DMEMϪ10% FBS. Media were collected after a 48-h incubation period.
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